Journal: bioRxiv
Article Title: Identifying Modulators of the Post-Antibiotic Effect
doi: 10.1101/2025.04.06.647494
Figure Lengend Snippet: Wild-type (black) and Δ rfaE (red) protein turnover of LpxC. The relative isotope abundance (RIA) was determined as a function of time after bacteria cultures were transferred to “heavy” lysine-containing media from “light” lysine media. The RIA was calculated by quantifying the isotopic abundance of peptides generated by the trypsin digest using mass spectrometry. Bacteria lysates were enriched using antibody pull-down with beads coated with the LpxC antibody from LSBIO. Experiments were performed independently in triplicate. Data were fit to an exponential decay giving rate constants of 0.078 (95% CI 0.062-0.098) and 0.041 (95% CI 0.035-0.050) min -1 for ecLpxC in the wild-type and Δ rfaE strains, respectively.
Article Snippet: The LpxC antibody was purchased from LSBIO.
Techniques: Bacteria, Generated, Mass Spectrometry